Sil Lab - Junior​/Assistant

Overview

The Sil lab in the School of Medicine – Microbiology and Immunology is seeking a highly‑motivated and detail‑oriented Junior/Assistant Specialist to support research in the basic biology of fungal pathogens. The candidate will work with postdoctoral fellows and other lab members to investigate novel biology in fungal development using molecular approaches, including genetics, genomics and RNA biology.

• Conduct molecular and cell biology experiments (PCR, qPCR, in vitro transcription, cloning, gel electrophoresis, immunoprecipitations, Northern and Western blotting)
• Aseptically culture fungal cell lines and perform genetic manipulations using CRISPR‑Cas9
• Prepare RNA and DNA libraries for next‑generation sequencing
• Perform microscopic analysis of fungal cells
• Collect, analyze, present experimental data

• Excellent organizational and interpersonal communication skills (verbal and written)
• Willingness and ability to learn new methods and skills for changing research priorities
• Ability to work independently and as a member of a research team
• Ability to prioritize tasks, coordinate work with others, and meet multiple deadlines
• Must have (or be in process of obtaining) a bachelor’s degree (or equivalent) or four years of research experience to be appointed at the Junior rank
• Must have (or be in process of obtaining) a master’s degree (or equivalent) or a bachelor’s degree with three or more years of research experience to be appointed at the Assistant rank

• Prior experience with pathogenic microbes, aseptic techniques
• Prior experience with BSL2/BSL3 biocontainment
• Prior experience designing and assembling DNA constructs
• Prior experience in molecular genetics and biology (gene knockouts, epitope tagging, immunoprecipitation, protein–protein interactions)
• Prior experience with RNAseq and library preparation
• Familiarity with RNA biology, gene expression assays, Western blotting
• Prior experience working in a research lab with fungi

Seeking at least a 2‑year commitment. Depending on performance and workload, opportunities for independent research, mentorship of trainees, and scientific writing will be available.

San Francisco, CA

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.