Junior Specialist: Brain Tumor Microenvironment Research

Junior Specialist - Aghi Lab

The Aghi Lab is seeking Junior Specialists who will participate in basic science research to study mechanisms by which brain tumors interact with their microenvironment to promote growth, invasion, and therapeutic resistance. Drug(s) to disrupt these processes will be evaluated in collaboration with industry partners in cell culture and in mouse models. Job duties will include culturing tumor cells; extraction of RNA and DNA form brain tumor tissue;

cloning retroviral constructs and isolating and purifying retroviruses from infected cells; immunohistochemistry and immunofluorescence of tumor tissue and cultured primary tumor cells; isolation of cells from brain tumors and flow cytometry of the cells to count numbers of various cell types; isolation of stem cells from brain tumors; CRISPR techniques; and intracranial and subcutaneous implantation of tumor cells into mice, followed by treatment and monitoring of these animals and euthanasia when appropriate.

The candidates will also handle lab administration - order entry, making sure protocols are in compliance, and orienting new lab members. Other duties as assigned.

Position Description

Junior Specialist - Aghi Lab

The Aghi Lab is seeking Junior Specialists who will participate in basic science research to study mechanisms by which brain tumors interact with their microenvironment to promote growth, invasion, and therapeutic resistance. Drug(s) to disrupt these processes will be evaluated in collaboration with industry partners in cell culture and in mouse models. Job duties will include culturing tumor cells; extraction of RNA and DNA form brain tumor tissue;

cloning retroviral constructs and isolating and purifying retroviruses from infected cells; immunohistochemistry and immunofluorescence of tumor tissue and cultured primary tumor cells; isolation of cells from brain tumors and flow cytometry of the cells to count numbers of various cell types; isolation of stem cells from brain tumors; CRISPR techniques; and intracranial and subcutaneous implantation of tumor cells into mice, followed by treatment and monitoring of these animals and euthanasia when appropriate.

The candidates will also handle lab administration - order entry, making sure protocols are in compliance, and orienting new lab members. Other duties as assigned.



Required Qualifications

• Specialists appointed at the junior rank must possess (or in process of obtaining) a baccalaureate degree (or equivalent degree) or must have at least four years of research experience.
• Applicants must have obtained the degree requirement for the Specialist rank by the time of hire.
• Experience with basic wet lab research (cell culture, pipetting, sectioning tissue, immunostaining, qPCR, mouse tumor implantation, western blotting).
• Ability to learn more advanced wet lab techniques (CRISPR, immunoprecipitation, purification of histidine-tagged proteins).
• Ability to independently plan experiments from start to finish to address a specific topic, ultimately leading the person filling this position to write portions of manuscripts.
• Ability to handle lab administration, including order entry, making sure protocols are in compliance, and orienting new lab members.
• Applicant's materials must list qualifications upon submission.

• Independent specialized research.

• Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).
• Cover Letter (Optional)
• Statement of Research (Optional)
• Statement of Teaching (Optional)

• 3 required (contact information only)