Scientist II, in vivo Gene Insertion

Responsibilities

• Design and execute workflows to conceive, build, and assess in primary cells.
  • Apply advanced cloning methods; handle diverse primary cell types (including T cells); support delivery approaches from transfection to viral transduction to targeted LNP (tLNP).
  • Collaborate across CRISPR Tx groups and external vendors to advance editing and delivery toward clinical readiness.
  • Use quantitative biology to iterate on editing efficiency/gene insertion, impacts on cell phenotype/construct expression, and new strategy ideation.
• Design and execute ddPCR assays to quantify transgene integration efficiency.
• Design and execute T cell phenotyping assays (e.g., multicolor flow cytometry panels).
• Leverage immunology and/or synthetic immunology to drive innovative editing, delivery, and expression strategies.
• Cross-functionally assess editing outcomes in animal models.
• Analyze and present experimental data to broad audiences.
• Generate, manage, evaluate, and maintain critical data with statistical analysis and troubleshooting.

• Extensive prior experience with gene editing and synthetic immunology (academic and/or industry) with a track record of significant contributions.
• Degree in Biology, Immunology, Genetics, Bioengineering, or related field (PhD: 2-5+ years; non-PhD: 10-12+ years relevant experience).
• Strong technical skills: cloning, flow cytometry, primary T cell culturing, editing assays, and T cell phenotype/function assessment.
• Ability to manage multiple priorities and support other projects.
• Strong communication and collaborative teamwork.

• Experience with in vivo delivery modalities (tLNP, AAV, VLP, etc.).
• Experience developing novel editing modalities.

Base pay range $,000+ plus bonus, equity, and benefits.