Research Associate, Research Scientist, Biotech Research

We are seeking a highly hands-on Research Associate or Scientist to support protein production and quality control workflows as we scale up for our customer's next phase of growth. Working alongside cross-functional scientists and engineers, you will execute, validate, and optimize protein expression, purification, and analytical characterization as part of an automated, high-throughput lab platform.

• Cell Culture & Expression: Run automated mammalian cell growth and transient transfection for recombinant protein expression, including cell maintenance, expansion, and scaling.
• Purification: Perform protein purification on AKTA (FPLC) and King Fisher platforms, including affinity capture, polishing, buffer exchange, and magnetic-bead-based processing.
• Analytical QC: Execute analytical QC by SEC (analytical UPLC/HPLC) to assess purity, aggregation, and monomericity, as well as supporting secondary QC (concentration, endotoxin, SDS‑PAGE).
• Automation
  
  Collaboration:
  
  Partner with roboticists and software engineers to translate manual purification and QC steps into reliable, autonomous protocols.
• Data Analysis & Documentation: Perform first-pass data analysis on chromatograms and SEC traces, maintaining rigorous experimental records in Electronic Lab Notebooks (ELN) and contributing to SOP development.

• B.S. or M.S. in Biochemistry, Molecular Biology, Chemical/Biological Engineering, or a related scientific discipline with hands‑on lab experience.
• Hands‑on experience personally running or developing 2+ of the following specific assays/instruments:

• -aSEC purity — Waters UPLC
• -Hydrophobic interaction (HI) — PAIA Biotech plate assay (Cytation readout)
• -OVA off‑target binding — Cytation readout
• -Thermal stability (nanoDSF) — Unchained Labs Aunty
• -Polydispersity (DLS) — Unchained Labs Aunty

• Direct hands‑on experience with protein purification (chromatography on AKTA/FPLC and/or bead‑based processing) and taking a sample from lysate/supernatant to purified protein.
• Strong wet‑lab skills, including mammalian cell culture, aseptic technique, and careful execution of detailed protocols.
• Basic understanding of antibody structure and function, strong documentation habits, and high attention to detail.

• Experience with analytical SEC or other analytical chromatography (UPLC/HPLC).
• Experience with automated or high‑throughput purification (e.g., King Fisher, multi‑column AKTA, robotic systems).
• Experience scaling mammalian expression systems (CHO/HEK) and transient transfection.
• Experience with lab automation or liquid handling platforms (e.g., Dynamic Devices Lynx, Tempest).
• Familiarity with alternative binder formats (VHHs, bispecifics, Fc‑fusions, or non‑Ig scaffolds).
• Basic scripting or data‑handling skills (Python/R) for organizing and analyzing purification and QC data.