Scientist II, Cell Line Engineering

Scientist II, Cell Line Engineering

Big Hat is looking for an energetic and motivated individual to lead cell line engineering as a core platform capability in support of our pipeline discovery and early R&D efforts. This is a lab based role that will report directly to the head of protein sciences, work cross functionally with programs and discovery, and directly impact the identification and validation of next generation biotherapeutics engineered on the milliner platform.

At Big Hat we believe in titles that commensurate with skill set, relative organizational impact, and value contribution; more experienced candidates are encouraged to apply, with the understanding that responsibilities and title would adjust as appropriate.

Department:
Lab Science

Employment Type:

Full Time

Location:

San Mateo, CA

Workplace Type:
Onsite

This role's hiring manager:
Sayantan Mitra

• Onboard all the core components necessary for engineering cell lines for expressing pipeline targets including expression vectors, gene delivery methods, selective antibiotic concentration determination protocols, expression validation and cell sorting capabilities
• Culture and maintain a variety of cell types; generate diverse target cell lines including knock-outs, stable cell lines over expressing one or more targets, and reporter cell lines
• Perform the validation of cell line expression using flow and sequencing (Sanger or NGS) based methods.
• Generate bulk pool and single cell populations utilizing FACS to select for cell lines of various expression levels
• Maintain an organized cell line inventory with all relevant documentation
• Investigate target expression biology to recapitulate the most native conditions for expression.
• Work with end user stakeholders to confirm correct domain expression and associated sequences
• In collaboration with end user stakeholders, establish the workflows, timelines and deliverables for cell line engineering from request to hand-off
• Collaborate with automation engineers to automate routine cell culture workflows to increase efficiencies and throughput

• PhD in biology-related discipline with minimum of 3+ years industry experience, preferably in an environment utilizing cell lines for antibody discovery and engineering
• In depth molecular biology knowledge for plasmid construction and experience selecting expression vectors and promoter types
• Experience culturing various cell types, including adherent, suspension, shaker flask etc. High level of organization in handling multiple cell lines simultaneously for manipulation and maintenance
• Demonstrated hands-on success in using CRISPR/Cas9, transposon, or lentivirus based technologies
• Adept with fluorescence or magnetic-bead based cell sorting methods, and clonal selection and maintenance
• Prior use of automation for cell culture a plus

The salary estimated for this position is $145,000 - $165,000 + bonus + options + benefits. Compensation will vary depending on job-related knowledge, skills, and experience. Actual compensation will be confirmed in writing at the time of the offer.

What Big Hat offers:

• Range of health insurance plan options through Anthem and Kaiser (monthly credit if benefit waived)
• Dental, and vision coverage through Guardian
• Additional well-being benefits through Nayya, One Medical, Wagmo, Rula, and more
• 401(k) with company match
• DTO, two weeks of company-wide shutdown, and 12 company holidays
• Paid parental leave

Big Hat Biosciences designs safer, more effective biologic therapies for patients using machine learning and synthetic biology. Big Hat integrates a wet lab for high-speed characterization with machine learning technologies to guide the search for better antibodies. We apply these design capabilities to develop new generations of safer and more effective treatments for patients suffering from today's most challenging diseases. Big Hat is a Series B biotech outside San Francisco with a team-oriented, inclusive, and family-friendly culture.

Our broad pipeline of wholly-owned and partnered therapeutic programs span many disparate indications with high unmet need, such as cancer, inflammation, and infectious disease. Big Hat has raised >$100M from top investors, including Section 32, a16z, and 8VC.

Stage 7:
Offer (Pending References)

Stage 8:
References

Stage 9:
Hired

Stage 1:
Applied

Stage 2:
Initial Conversation | Head of Talent

Stage 3:
Initial Conversation | Hiring Manager

Stage 4: 1st Technical Screen

Stage 5: 2nd Technical Screen

Stage 6:
Full Panel Interview

Stage 7:
Offer (Pending References)

Stage 8:
References

Stage 9:
Hired

Not quite right? Register your interest to be notified of any roles that come along that meet your criteria.

Register Your Interest