Junior Specialist in Neuroscience Research Institute (Kosik lab

Junior Specialist in Neuroscience Research Institute (Kosik Lab)

The laboratory of Dr. Kenneth Kosik in the Neuroscience Research Institute at the University of California, Santa Barbara invites applications for a full-time Junior Specialist. The appointee will contribute to ongoing research in mammalian neuronal systems by establishing primary neuronal cultures from mouse embryos and pups, including dissociation of brain tissue and subsequent plating of neurons, as well as assisting with the routine maintenance of iPSC lines through passaging, media changes, and management of cryopreserved stocks.

Key Responsibilities:

• Contribute to the differentiation of iPSC lines into relevant neural cell types, including cortical neurons, astrocytes, and other lineages required by ongoing projects.
• Generate human brain organoids from iPSC lines while troubleshooting and optimizing established protocols.
• Prepare and perform systematic quality control of brain organoids and neural cell cultures through brightfield and fluorescence microscopy, cell viability assays, RT-qPCR, and immunocytochemistry.
• Assist in characterizing neuronal cultures and brain organoids immunocytochemically, communicating results and preparing reports.
• Support general laboratory operations related to the stem cell facility including reagent procurement, inventory management, and preparation of media and solutions.
• Maintain thorough and accurate experimental records and contribute to the presentation of results at laboratory meetings.

Qualifications

Applicants must have completed or be currently enrolled in a BA or BS program in biology or related field at the time of application.

• Applicants must have their bachelor's degree conferred by the start date of the position.
• Two to three years of cell culture experience at time of start.

The ideal candidate will have experience in:
• Cell culture
• Stem cells
• Molecular biology