Senior Scientist

Vibrant America is a leading clinical diagnostics laboratory dedicated to transforming healthcare through advanced testing, innovative technology, and data-driven insights. The company specializes in functional medicine and multi-omics testing, offering comprehensive panels that support personalized patient car

At Vibrant America, our vision is to empower healthcare providers and patients with actionable insights that drive better health outcomes. By integrating cutting-edge science, automation, and analytics, we aim to redefine preventive and personalized medici

The Senior Mass Spectrometry Scientist will lead the design, development, validation, and production deployment of targeted quantitative LC-MS/MS assays for metabolites and small molecules, which constitute approximately 80% of the role. Working primarily on triple-quadrupole platforms, the scientist will build robust, high-throughput quantitative methods for a broad range of analytes — including amino acids, organic acids, fatty acids, bile acids, vitamins, steroids and hormones, and other clinically relevant metabolites — across diverse biological matrices.

A core expectation of this role is taking assays from R&D through analytical and clinical validation and into the production / manufacturing pipeline of a high-complexity clinical laboratory. The remaining ~20% of the role supports targeted proteomic and peptide quantitation on high-resolution platforms as part of the company’s multi-omics testing portfolio. The scientist will collaborate cross-functionally with R&D, bioinformatics, automation, quality assurance, and clinical operations teams to advance projects from concept to live, patient-impacting te

• Develop, optimize, and validate targeted quantitative LC-MS/MS methods (MRM/SRM) for metabolites and small molecules across matrices such as plasma, serum, urine, and other biofluids.
• Design quantitation strategies using stable isotope-labeled internal standards, calibration curves, and robust QC schemes to achieve accurate, precise, and reproducible measurements.
• Develop and optimize sample-preparation workflows (protein precipitation, solid-phase extraction, liquid-liquid extraction, derivatization) using both manual and automated / liquid-handling platforms.
• Develop chromatographic separations (reversed-phase, HILIC) suited to chemically diverse small-molecule analytes.
• Lead the transfer of validated assays into the production / manufacturing line, partnering with operations and automation teams to ensure throughput, robustness, and long-term reproducibility.
• Perform analytical and clinical method validation (accuracy, precision, linearity, LOD/LOQ, matrix effects, carryover, stability, reference intervals) in accordance with CLIA/CAP and applicable regulatory standards.
• Support the development and execution of targeted proteomic / peptide assays (e.g., PRM, stable isotope dilution) on high-resolution Orbitrap and LC systems for multi-omics panels.
• Contribute to peptide mapping, post-translational modification (PTM) analysis, and intact / protein-level measurements as needed.
• Operate, maintain, calibrate, and troubleshoot triple-quadrupole LC-MS/MS systems (e.g., Sciex, Agilent, Waters) and associated UHPLC; work with high-resolution Orbitrap platforms for confirmation and proteomic applications.
• Perform routine performance checks, system suitability testing, and preventive maintenance to ensure data quality in a regulated environment.
• Coordinate with vendors to implement upgrades, new applications, and service support.

• Process and interpret quantitative MS data using vendor and specialized software (e.g., Sciex OS / Multi Quant, Agilent Mass Hunter Quantitative, Waters Target Lynx, Thermo Trace Finder, Skyline).
• Integrate metabolomics data with proteomics, genomics, and other omics layers to generate multi-dimensional, clinically meaningful insights.
• Collaborate with bioinformatics teams to refine quantitation pipelines, perform statistical analyses, and support clinically relevant reporting.

• Le…