Protein Sciences Research Scientist

Protein Sciences Research Scientist page is loaded## Protein Sciences Research Scientist locations:
Seattle, WAtime type:
Full time posted on:
Posted Todayjob requisition :
REQ-28792
** Job Description
**** General

Summary:

** We are seeking a motivated Scientist to join our Protein Science team. In this role, you will purify and characterize therapeutic proteins and research reagents at milligram- to gram-scale in support of early- and late-stage discovery programs. The ideal candidate will bring strong expertise in protein purification, protein analytics, and protein-protein interaction characterization, and will help advance platform capabilities through innovative approaches in analytical characterization and biologics develop ability assessment.
** Key

Duties and Responsibilities:

*** Operate AKTA chromatography systems using UNICORN software to purify antibodies, Fc fusion proteins, and His-tagged protein reagents for in vitro and in vivo applications.
* Design and execute fit-for-purpose purification strategies using Protein A, immobilized metal affinity chromatography (IMAC), ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC), and preparative size exclusion chromatography (SEC).
* Perform analytical characterization using UV-Vis spectroscopy, analytical SEC on HPLC/UPLC systems with Empower software, endotoxin testing, SDS-PAGE, and western blotting.
* Develop, implement, and improve analytical workflows to support the develop ability assessment of biologics.
* Collaborate with cross-functional teams and external partners to support mass spectrometry analyses.
* Develop and optimize bio-layer interferometry (BLI) assays to evaluate protein-protein interactions and determine binding kinetics, including KD, kon, and koff.
* Present purification, analytical, and develop ability data to the Protein Science team, project teams, and the broader research organization.
* Maintain accurate, timely, and well-organized records in electronic lab notebooks and protein inventory/database systems.
** Knowledge and

Skills:

*** Demonstrated expertise operating AKTA systems and UNICORN software for protein purification.
* Strong hands-on experience with protein capture and polishing methods using resins such as Mab Select SuRe, Mab Select Prism

A, Capto L, Capto DEAE, Capto Q, and Capto S.
* Proficiency with Waters HPLC/UPLC systems and Empower software, including SEC, HIC, IEX, and reversed-phase methods.
* Experience performing SDS-PAGE and developing or troubleshooting western blot methods.
* Experience evaluating protein stability and manufacturability using biophysical methods such as DSF, DLS, AC-SINS, cross-interaction chromatography (CIC), or standup monolayer chromatography (SMAC).
* Experience supporting protein therapeutic candidates from early discovery through advancement toward development in highly collaborative, fast-paced, and results-driven teams.
* Experience using BLI or SPR to perform kinetic or quantitative binding assays.
* Familiarity with cIEF for charged variant analysis and mass spectrometry for protein identification and post-translational modification (PTM) analysis.
*
* Education and Experience:

*** PhD in Cell Biology, Biochemistry, Biophysics, Chemical Engineering, Bioengineering, or a related field with 2+ years of relevant industry experience; or
* Master’s degree in one of the above fields with 3+ years of relevant industry experience; or
* Bachelor’s degree in one of the above fields with 6+ years of relevant industry experience.
** Pay Range:**$104,000 - $156,000
** Disclosure Statement:
** The range provided is based on what we believe is a reasonable estimate for the base salary pay range for this job at the time of posting. This role is eligible for an annual bonus and annual equity awards. Some roles may also be eligible for overtime pay, in accordance with federal and state requirements. Actual base salary pay will be based on a number of factors, including skills, competencies, experience, and other job-related factors permitted by law.

At Vertex, our Total Rewards offerings also include inclusive market-leading benefits to meet our employees wherever they are in their…