Flow Cytometry Technician

Key Responsibilities

• Flow cytometers Maintenance:
  Operate and maintain a diverse suite of analytical instruments (including BD Symphony A5, BD LSR Fortessa, Bio-Rad ZE5, and Beckman Coulter Cytoflex) to ensure peak performance and user readiness.
• Sorter Operation & Service:
  Independently operate, calibrate, and provide expert cell sorting services using advanced cell sorters (e.g., BD S6, Bio-Sorter).
• Quality Control (QC):
  Perform daily QC, preventative maintenance, and tracking for all core instrumentation; maintain detailed, accurate logs and documentation.
• Troubleshooting:
  Execute first-level instrument troubleshooting to resolve common hardware and software issues. Escalate complex instrument failures to the Core Leader and coordinate service visits with external vendors.
• Compliance & Safety:
  Ensure strict adherence to Core Standard Operating Procedures (SOPs) and institutional safety protocols, including EH&S and Biosafety Level 2 (BSL2+) regulatory requirements.
• Requires some physical activity, including equipment setup, reagent handling, and light lifting (up to 50 lbs.)

• Education:
  
  Bachelor’s degree in Biology, Immunology, Molecular Biology, Biochemistry, or a related scientific field.
• Flow Cytometry Expertise:
  Significant hands‑on experience in multi‑color flow cytometry, including sample preparation, data acquisition, and data analysis using software such as Flow Jo, FCS Express, etc.
• Cell Sorting
  
  Experience:
  
  Demonstrated proficiency in the independent operation, calibration, and maintenance of cell sorters to provide reliable cell sorting services.
• Technical Literacy:
  Familiarity with standard computer systems, literature search methodologies, and data plotting/statistical analysis.
• Prior experience in spectral flow cytometry and imaging technologies is highly preferred.
• Excellent verbal and written communication, organizational, and record‑keeping skills. Proven ability to work independently, adaptable team player committed to supporting a collaborative research environment.

Bachelor’s Degree in an Engineering or Scientific discipline. Experience in a laboratory/research setting. Demonstrated knowledge in solving technical problems such as issues with lab/flow cytometry instrumentation.

N/A.

• Experience acquiring and analyzing Flow Cytometry data, preferably with BD Facs Diva software.
• Data entry and/or word processing experience.
• Experience in analyzing data using spreadsheets and statistical analysis.

$23.15 - $34.73 per hour.

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more.

Seattle Children’s welcomes people of all experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our patients and families. Our organization recruits, employs, trains, compensates, and promotes based on merit without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

Seattle Children’s is proud to be an Equal Opportunity Workplace and Assertive Action Employer.