Research Scientist​/Engineer - Department of Medicine, Division of Hematology & Oncology

Research Scientist

The Department of Medicine, Division of Hematology & Oncology has an outstanding opportunity for a Research Scientist to join their Cancer Vaccine Institute (CVI) team.

Reporting to the Research Scientist Senior at the Cancer Vaccine Institute (CVI), the Research Scientist 3 is responsible for promoting the research objectives of the UW CVI in the Division of Hematology & Oncology. This professional research position works directly with clinical and laboratory-based faculty members who specialize in translational immunotherapy research targeting a variety of cancers. The Research Scientist will play a key role in implementing clinical trials that test new cancer therapies by generating reliable and interpretable laboratory data from clinical research specimens to support evaluation of immune responses, treatment-related biomarkers, and mechanisms of response or resistance.

Working under general guidance, the Research Scientist 3 will select and apply standardized procedures and techniques in the development, execution, validation, and troubleshooting of immunologic and translational biomarker assays used in cancer vaccine and immunotherapy clinical trials.

The Research Scientist works on-site at the UW Medicine at South Lake Union campus.

• Laboratory Operations and Assay Execution (65%)
• Perform skilled immunologic assay execution and serve as an experienced scientific and technical resource in identifying issues and problem-solving approaches.
• Ensure reagent and equipment qualification. Prepare and maintain laboratory reagents, chemicals, instruments and supplies. Order reagents and keep track of laboratory supplies, reagent lots, and inventory.
• Process, isolate, cryopreserve, thaw, count, and assess PBMC and/or other immune cell populations from clinical research blood.
• Perform cytokine ELISpot / Fluoro Spot assays
• Perform multiparameter flow cytometry and identify cytokine-producing T cells using intracellular staining.
• Stimulate and culture primary cells in vitro for up to several months
• Conduct ELISA/Luminex assays to measure cytokine production
• Perform lymphoproliferative assays and cytolytic killing assays
• Perform nucleic acid purification from blood, PBMCs, tissue, FFPE specimens, and other biospecimens.
• Support NGS-related sample preparation, including nucleic acid QC, library preparation workflows, and sample preparation for sequencing or external vendor submission.
• Support tissue-based and spatial omics sample preparation, including FFPE/frozen tissue handling, slide preparation, and specimen preparation for multiplex or spatial biomarker assays.
• Assay Development, Optimization, and Quality Support (15%)
• Contribute to the development, optimization, qualification, validation, and implementation of new or modified laboratory procedures and assays. Integrate new ideas, procedures, and routines as necessary.
• Support assay troubleshooting, development, reagent lot comparison, method bridging, equipment qualification, and assay performance review.
• Utilize broad knowledge of principles, practices, and procedures to develop, write and revise SOPs, worksheets, forms, sample handling instructions, and technical documents.
• Data Collection, Analysis, and Reporting (10%)
• Maintain accurate records of sample receipt, processing, assay setup, reagent lots, equipment use, QC results, deviations, and data analysis.
• Collect, review, analyze, and interpret results. Identify technical issues and recommend procedure modifications as needed.
• Prepare data summaries, reports, tables, figures, and technical updates for laboratory leadership, collaborators, and study teams.
• Communication, Collaboration, and Technical Guidance (10%)
• Interact with investigators, clinical teams, coordinators, external collaborators, vendors, and clinical trial sites regarding sample collection, processing, shipping, testing, and data deliverables.
• Contribute to positive workplace relationships through professional, efficient, and timely interactions on current assignments and projects.
• Serve as an experienced technical resource/mentor, monitor the work of others, and provide technical guidance, coaching, or training support to junior staff, trainees, fellows, or collaborators as appropriate.

To be considered for this opportunity your application must demonstrate you meet both the minimum qualifications and additional qualifications listed below. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Minimum Qualifications

• Bachelor's degree in biology, immunology, molecular biology, or related field and four years of experience in a clinical research setting.

Additional Qualifications

• Significant hands-on experience in cellular immunology, immune monitoring, tissue culture, and clinical research sample handling.
• Experience with PBMC processing, cryopreservation/thawing, flow cytometry,…