Research Technician III

Overview

Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington.

With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world's leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states.

Together, our fully integrated research and clinical care teams seek to discover new cures to the world's deadliest diseases and make life beyond cancer a reality.

At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems.

This research opportunity is with the McGuire Laboratory, in the Vaccine and Infectious Disease Division at the Fred Hutchinson Cancer Center.

Our lab is recruiting a Research Technician III to support our efforts to develop vaccines against pathogens of public health importance such as EBV, KSHV, and RSV.

The technician will be expected undertake several roles in a highly collaborative environment. Responsibilities include carrying out immunizations in small animal models and evaluating vaccine responses to different vaccine antigens using ELISA and flow-cytometry based viral neutralization assays, as well as T cell assays including intercellular cytokine staining and ELISPOT. The role may require cell line development using lentiviral transduction, confirmation of transgene expression by immunofluorescence microscopy and in vivo imaging of transferred cells using IVIS.

In addition, the role will require maintaining cell cultures and general lab upkeep.

The candidate will be responsible for performing and coordinating the assays with other lab members and analyzing the data. They will interpret and report on experimental results and assist with data presentation. They will assist in writing laboratory SOPs, reports, and other documentation. They will report to a Staff Scientist, the Principal investigator and work closely with a post-doctoral scientist.

• Maintain a high level of accuracy for all work.
• Work well as team-member, participate in positive communication, and foster an environment that allows all laboratory staff to work well together.
• Maintain cell cultures and produce viral stocks for conducting neutralization experiments
• Conduct immunogenicity studies in mice and analyze immune responses
• Proactively prepare reagents for experiments and for general lab use.
• Carry out maintenance, troubleshooting and QC of laboratory equipment.
• May lead or supervise other laboratory personnel and be responsible for the daily operation of the laboratory.
• Collaborate with primary investigator, and assist in designing experiments; examine proposals to determine projects and methodologies best suited to research objectives and the overall goals of the laboratory.
• Summarize and write for publication or presentation the results of research projects, often as co-author or author. Edit and write research reports.
• Conduct literature reviews to assist in determining most suitable methods to be used in research.
• Other duties as assigned.

• Bachelor's degree in related scientific field or equivalent combination of education and experience.
• 5 years of experience working in a lab outside of school coursework (or equivalent combination of advanced degree and work experience).

• Specific experience working with mice.
• Experience with serological and…