Scientist - Functional Proteomics

Scientist
- Functional Proteomics

Location:

Seattle, WA

Compensation: $115,000-$150,000 annual base salary

Employment Type:

Direct Hire

Work Arrangement:
Primarily onsite

Yoh is proactively seeking a Scientist
- Functional Proteomics for an anticipated opportunity with a biotechnology company in Seattle, WA.

We are looking for a hands-on scientist with expertise in mass spectrometry-based proteomics who can design rigorous quantitative experiments and translate complex proteomic data into meaningful biological insights. This position will support drug-discovery research involving target identification and validation, compound characterization, and mechanism-of-action studies.

The ideal candidate combines strong experimental proteomics capabilities with a deep understanding of protein biology and the ability to collaborate across experimental biology, computational science, and drug-discovery teams.

• Design and execute quantitative and functional proteomics experiments supporting target discovery, target validation, compound profiling, and mechanism-of-action research.
• Develop and optimize protein extraction, digestion, enrichment, fractionation, and sample-preparation workflows for mass spectrometry-based studies.
• Design studies evaluating protein abundance, activity, binding, localization, and responses to compound treatment.
• Establish appropriate experimental controls, biological and technical replicates, normalization strategies, and quantitative approaches.
• Develop proteomic strategies for challenging protein classes, including transcription factors, chromatin-associated proteins, and other regulatory proteins.
• Perform mammalian cell culture and cellular compound-treatment experiments as needed.
• Generate, analyze, and interpret quantitative proteomics datasets in collaboration with computational scientists.
• Integrate proteomic findings with genomic, transcriptomic, cellular, and pharmacological datasets to develop biological conclusions.
• Troubleshoot experimental workflows and improve sensitivity, reproducibility, robustness, and throughput.
• Support the development of automated and high-throughput proteomics workflows.
• Present experimental findings, scientific interpretations, and recommendations to cross-functional project teams.
• Contribute to scientific strategy, technical documentation, publications, and presentations.

• Ph.D. in proteomics, biochemistry, molecular biology, chemical biology, analytical chemistry, systems biology, or a related scientific discipline preferred.
• Hands-on experience with mass spectrometry-based proteomics.
• Strong understanding of quantitative proteomics experimental design, including sample preparation, controls, replicates, and normalization.
• Experience generating, processing, and/or interpreting quantitative proteomics datasets.
• Strong foundation in protein biology and hypothesis-driven experimental science.
• Demonstrated ability to translate complex datasets into meaningful biological conclusions.
• Ability to independently troubleshoot experimental workflows and make scientifically sound decisions.
• Strong communication skills and experience collaborating across multidisciplinary scientific teams.

• Functional proteomics or chemoproteomics
• Compound-target identification or target-engagement studies
• Target discovery, target validation, or mechanism-of-action research
• Thermal proteome profiling or other protein-stability approaches
• Affinity-based or enrichment-based proteomic methods
• DIA, label-free, isotopic, or multiplexed quantitative proteomics
• Nuclear, chromatin, or transcription-factor proteomics
• Mammalian cell culture and compound-treatment studies
• Drug discovery or pharmacology
• Automated or high-throughput proteomics…