Staff Research Associate II-(SRA2H

Position: Staff Research Associate II-(SRA2H)
About Us:

The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center is an independent, non-profit biomedical research organization located in Torrance, California. In dozens of laboratories throughout the campus, researchers are working to unravel the mysteries of heart and kidney disease, cardiac complications in newborns, chronic lung disease, skin cancer, sickle cell disease, disorders of the autoimmune system, and much more. Many of these projects are collaborative in nature, involving experts from different areas of specialization.

Our discoveries have prevented blindness in newborns, enabled premature infants with fragile lungs to breathe and allowed children with rare, often fatal diseases to grow up healthy and strong.

Position Summary

The Al Alam Laboratory at The Lundquist Institute is seeking a highly motivated Research Associate II to support NIH- and CIRM-funded studies focused on modeling human lung development and disease using induced pluripotent stem cells (iPSCs). The successful candidate will contribute to innovative research investigating the cellular and molecular mechanisms underlying alveolar hypoplasia in Down syndrome through the generation, differentiation, and characterization of human iPSC-derived alveolar epithelial cells and organoid systems.

The position offers opportunities to work at the intersection of developmental biology, regenerative medicine, stem cell biology, genomics, and lung disease research. The candidate will work closely with a multidisciplinary team of investigators, trainees, and collaborators and will play a central role in advancing translational research projects.

Essential Duties and Responsibilities

Maintain and expand human induced pluripotent stem cell (iPSC) cultures under feeder-free conditions.
Perform reprogramming of primary human cells into iPSCs using non-integrating approaches such as Sendai virus vectors.
Differentiate human iPSCs into lung progenitor cells and alveolar epithelial type II (iAT2) cells following established directed differentiation protocols.
Establish and maintain three-dimensional organoid cultures, alveolosphere cultures, and air-liquid interface (ALI) systems.
Conduct routine characterization of pluripotent and differentiated cells using flow cytometry, immunofluorescence, confocal microscopy, RT-qPCR, and other molecular assays.
Perform cell sorting and isolation using flow cytometry or magnetic bead-based approaches.
Assist with CRISPR/Cas9-based gene editing, gene knockdown, and over-expression studies.
Prepare samples for bulk RNA sequencing, single-cell/single-nucleus sequencing, ATAC-seq, and multiomic analyses.
Maintain accurate electronic laboratory records and contribute to experimental design, data analysis, figure preparation, and manuscript development.
Assist with laboratory management activities, including ordering supplies, maintaining inventories, and ensuring compliance with laboratory safety procedures.
Participate in laboratory meetings, project discussions, and collaborative research activities.

Minimum Qualifications

Master's degree in Cell Biology, Molecular Biology, Stem Cell Biology, Biomedical Sciences, Biotechnology, or a related field.
Minimum of one-two years of hands-on research experience in an academic, biotechnology, pharmaceutical, or biomedical research environment.
Demonstrated experience culturing human pluripotent stem cells (iPSCs and/or embryonic stem cells).

Experience with sterile mammalian tissue culture techniques.
Proficiency in molecular biology methods including RNA isolation, qPCR, cDNA synthesis, and immunostaining.
Strong organizational skills and ability to manage multiple projects simultaneously.
Excellent written and verbal communication skills.

Preferred Qualifications

Experience with directed differentiation of iPSCs into lung, endodermal, epithelial, or organoid models.

Experience with alveolar epithelial cell culture, lung biology, developmental biology, or regenerative medicine research.
Familiarity with flow cytometry and fluorescence-activated cell sorting (FACS).
Familiarity with confocal microscopy and advanced image analysis software.
Experience…