Research Technician III, Cellular and Molecular Medicine

Research Technician III, Cellular and Molecular Medicine (Full-Time)

Posting Number req
27112

Department Cellular & Molecular Medicine

Department Website Link (Use the "Apply for this Job" box below)./

Location Tucson Campus

Address 1501 N. Campbell Ave., Tucson, AZ 85719 USA

The Department of Cellular and Molecular Medicine is seeking a highly skilled Research Technician III to support advanced laboratory operations with minimal supervision. The ideal candidate will bring specialized expertise in culturing human endothelial cells and molecular biology techniques and will demonstrate the ability to troubleshoot independently and propose innovative solutions to experimental challenges.

• Visa sponsorship is not available for this position.

Outstanding U of A benefits include health, dental, and vision insurance plans; life insurance and disability programs; paid vacation, sick leave and holidays; UA/ASU/NAU tuition reduction for the employee and qualified family members; state retirement plan; access to UA recreation and cultural activities; and more!

Perform complex experimental procedures, including:

• Growth of human endothelial cell cultures in single and pooled cultures.
• Exposing cultured cells to shear stress to mimic hemodynamic blood flow.
• Performing molecular biology techniques such as nucleic acid extractions, quantitative real-time PCR, RNA-seq, ATAC-seq, ChIP-seq, and other
  -seq assays including library preparation.
• Performing immunolabeling, immunoblotting, and confocal/high-content microscopy for cellular imaging.
• Transfect/infect cells with oligos and lentivirus in vitro for gain- and loss-of-function experiments.

• With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments.
• Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs).

• Communicates effectively all aspects of experiments with PI or other lab members and collaborators.
• Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases and file systems.
• Communicates with collaborating investigators and laboratories.
• Catalogs specimens and samples.
• Performs independent analysis and interpretation of data for investigator and team.

• Orders and catalogs laboratory supplies and supports laboratory operations.
• Assist in maintaining laboratory equipment and related records.
• Operates and calibrates specialized scientific equipment including instruments for exposing cell cultures to fluid shear stress.

• Ability to maintain protocols, data, and samples organized with clear documentation recording how experiments were performed to ensure rigor and reproducibility.
• Ability to communicate clearly with all members of the team.

This job posting reflects the general nature and level of work expected of the selected candidate(s). It is not intended to be an exhaustive list of all duties and responsibilities. The institution reserves the right to amend or update this description as organizational priorities and institutional needs evolve.

• High school diploma or high school diploma equivalency is required.
• Minimum of 5 years of relevant experience, or equivalent combination of education and work experience.

• Bachelor's degree in Life Sciences.
• Prior experience in experimental laboratory work, mammalian cell culture, buffer and reagent preparation, and sterile technique.
• Prior experience with microscopy.

FLSA Non-Exempt

Full Time/Part Time Full Time

Number of Hours Worked per Week 40

Job FTE …