Associate Scientist​/Principal Associate Scientist, Protein Analytics

Overview

Alloy Therapeutics — a biotechnology ecosystem company empowering the global scientific community to make better medicines together. Alloy democratizes access to pre-competitive tools, technologies, services, and company creation capabilities for discovering and developing therapeutic biologics. Alloy is headquartered in Waltham, MA with additional labs in Cambridge, UK;
Basel, Switzerland; and Athens, GA. The company supports a culture of accountability, deliberate trust, and open communication, aiming to maintain a nimble, startup environment.

This is an on-site and full-time position in Waltham, MA. Alloy operates with an ethos of mentorship and prefers workers in the office when needed for interaction. As a lab-based operation, the team works daily in the lab. We value colleagues who have varying flexibility and emphasize being one team.

Alloy s Protein Analytics group, within the Alloy Antibody Powered Division, provides analytical support across discovery and development activities, including assay development, biophysical characterization, high-throughput screening, and quality control of monoclonal and multispecific antibodies, TCRs, TCR mimics, and other modalities.

We are seeking a highly motivated Associate Scientist or Principal Associate Scientist to conduct in vitro screening and analytical characterization as part of a fast-paced biotech team. You will design and execute LC-MS experiments, purity assessments, recombinant protein-binding screens, and develop ability evaluations. You will work collaboratively to advance biologics discovery and support data-driven decision-making.

This role reports to the Head of Protein Analytics and offers significant autonomy, with ownership of multiple stakeholders, projects, and tasks in a dynamic start-up environment while maintaining Alloy standards.

• Execute: Perform LC-MS based characterization of antibodies, purity assessments, protein binding, and develop ability characterization.
• Develop: Optimize experimental workflows for efficient data generation.
• Deliver: Support discovery and development projects for monoclonal and multispecific antibodies, and TCR-mimic antibodies.
• Collaborate: Work across multi-disciplinary teams in biologics discovery programs across Alloy sites.
• Communicate: Clearly convey scientific concepts, experimental results, and interpretations to internal and external stakeholders.

• Design and conduct LC-MS based experiments.
• Develop new methods and optimize existing workflows.
• Perform purity assessments (SEC-HPLC, CE-SDS, etc.).
• Maintain analytical instrumentation and ensure best practices.
• Support protein binding assays using BLI and SPRi.
• Support develop ability assessments utilizing in vitro methods.
• Present experimental data and findings during team meetings.
• Ensure timely and successful execution of project activities according to plan.
• Maintain detailed laboratory records in compliance with industry standards.
• Maintain laboratory organization and ensure safety compliance.

• B.S. and M.S. in Biochemistry or related field, with 2-6 years of relevant industry experience.
• Proven track record of expertise in protein analytical and/or biophysical assessments.
• Hands-on experience with LC-MS, SEC-HPLC, CE-SDS, and related methodologies.
• Experience in maintenance and troubleshooting of analytical instrumentation.
• Preferred experience with protein-binding (BLI, SPRi) and/or cell-binding (Flow Cytometry).
• Preferred experience with protein labelling, including biotinylation and fluorophore conjugation.
• Demonstrated ability to troubleshoot and optimize analytical instrumentation.
• Excellent written and verbal communication skills, with ability to present complex scientific data.
• Ability to multitask and deliver results within project-driven timelines.
• Strong sense of ownership, urgency, persistence, and scientific integrity.
• Excellent organization, clear communication, and teamwork in a multidisciplinary setting.
• Thrives in a team environment and collaborates to achieve project goals.
• Detail oriented with rigorous scientific thinking and a passion for drug discovery to…