Senior Research Associate Strain and Enzyme Engineering

Research Associate Senior Research Associate Strain and Enzyme Engineering Waltham MA Manus works across industries and value chains to accelerate the transition to Bio Alternatives better performing and more sustainable versions of complex molecules traditionally sourced from plants animals or fossil fuels Our platform is proven to work across scales bridging the Valley of Death between lab and manufacturing more efficiently and more reliably to deliver the benefits of biotechnology today Manus is seeking a Research Associate RA or Senior Research Associate SRA to support strain engineering and analytics workflows within our project pipeline In this role you will design and construct plasmids generate chromosomally integrated Escherichia coli E coli strains culture and screen engineered strains and process the resulting analytical data liquid extraction Gas Chromatography GC andor Liquid Chromatography LC to identify strains with enhanced performance This role focuses on execution of defined engineering campaigns to drive meaningful improvements in a fast paced cross functional environment This hands on position requires a desire and ability to work as an integral part of a multidisciplinary team as well as the demonstration of a high degree of flexibility creativity and initiative This person will be expected to play an integral part of Manuss research and development team located in Waltham Massachusetts MA

• Molecular biology Design construct and validate plasmids for gene expression and pathway engineering in microbial hosts
• Strain construction Generate chromosomally integrated E coli strains eg via recombineering or CRISPR based methods to support strain engineering campaigns
• Strain cultivation Culture and maintain engineered E coli strains in solid and liquid formats at lab scale
• Sample preparation Perform liquid liquid or solid phase extraction of fermentation broth and other sample matrices to prepare samples for analytical testing
• Analytical execution Operate GC andor LC instrumentation to generate quantitative data on strain performance eg titer yield product profile
• Data analysis Process QC and interpret analytical and strain performance data; summarize and present findings to the project team
• Data management Ensure accurate data collection processing and upload to LIMS systems
• Collaboration Collaborate with cross functional teams molecular biology fermentation downstream processing to improve experimental outcomes
• Lab operations Maintain instrumentation readiness and general lab organization
• Documentation Author and update SOPs and experimental documentation

• Research Associate Bachelors degree with 04 years relevant experience
• Senior Research Associate Bachelors with 47 years or masters with 24 years relevant experience
• Degree in molecular biology biochemistry microbiology chemistry bioengineering or a related field field of degree is flexible; relevant hands on experience is prioritized
• Hands on experience with plasmid design and cloning eg Gibson assembly Golden Gate restrictionligation
• Experience culturing and screening engineered microbial strains in liquid andor solid formats

• Direct experience generating chromosomal integrations in E coli specifically eg lambda Red recombineering CRISPR based integration
• Experience with LIMS systems eg Benchling
• Data analysis and visualization experience eg JMP Python SQL
• Familiarity with GC MS or LCMS mass selective detection in addition to GC FID or standard LCUV Vis workflows
• Experience with sample extraction liquid liquid or solid phase from biological or fermentation matrices
• Experience with automated liquid handling systems

Ability to work in industrial environments

May require standing walking climbing stairs and lifting equipment up to 50 lbsUse of PPE required in designated areas