Research Technologist

Job Overview

Job : 53164
Location: Chicago, Illinois

Department: MED-Ophthalmology
Salary/Grade: NEX/9

Target hiring range for this position will be between be range is as be $19.43-$20.59 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

Conducts tests and procedures; sets up and operates equipment; records observations and measurements, and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques.

• Follows pre-defined protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices, and conducts standard and repetitive experiments and completes basic troubleshooting techniques.
• Sets up, operates, and maintains standard lab equipment.
• Using an entry level of skill, complete the following activities:
• Records test results and may maintain databases.
• Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
• Maintains inventory of reagents, mice, and lab supplies
• Molecular Biology: DNA, RNA, Protein isolation, purification, and expression Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping, and sequencing of DNA
• Recombinant virus production and purification
• Cellular Biology:
  Cell culture, cell isolation
• Functional studies
• Transfection
• Histology:
  Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry
• Microscopy Immunology:
  Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification
• Animal:
  Husbandry
• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony
• Miscellaneous:
  Performs other duties as assigned.

• HS diploma or equivalent, and some basic science courses are required.

• Research experience using Excel and data tables. Ability to write experimental protocols.

• College degree in computer science, engineering, or a biological science.

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