Research Biologics Technologist 1

Shirley Ryan Ability Lab is the global leader in physical medicine and rehabilitation for adults and children with the most severe, complex conditions. By joining our team, you’ll be part of our life-changing mission and vision. You’ll contribute to an innovative, multifaceted culture that is second to none — one that embraces collaboration, excellence, discovery and compassion. You’ll play a role in something that’s never been done before as we integrate science and clinical care to help patients achieve better, faster outcomes — as we Advance Human Ability, together.

Job Description Summary The Research Biologics Technologist 1 will work in a team structure to support the development and implementation of the lab.

The Research Biologics Technologist 1 will consistently demonstrate support of the Shirley Ryan Ability Lab statement of Vision, Mission and Core Values by striving for excellence, contributing to the team efforts and showing respect and compassion for patients and their families, fellow employees, and all others with whom there is contact at or in the interest of the institute.

The Research Biologics Technologist 1 will demonstrate Shirley Ryan Ability Lab Core Attributes:
Communication, Accountability, Flexibility/Adaptability, Judgment/Problem Solving, Customer Service and Core Values (Hope, Compassion, Discovery, Collaboration, and Commitment to Excellence) while fulfilling job duties.

Job Description Principal Responsibilities Technical:

Follows pre-defined protocols, policies, procedure, regulations, Good Clinical & Good Laboratory Practices & conducts standard & repetitive experiments & completes basic trouble-shooting techniques.

Sets-up & operates & maintains standard lab equipment.

Administrative:

Records test results & may maintain databases and perform some basic statistical analyses.

Completes standard & routine clerical duties such as copying, filing, data entry, word processing, etc.

Maintains inventory of reagents, mice & lab supplies.

Skills:

Using an entry level of skill, completes the following activities:

Molecular Biology and Biochemistry:

DNA, RNA, Protein isolation, purification & expression

Southern, Northern, Western blottingPCR, RT-PCR,Plasmid preparation

Cloning, mapping & sequencing of DNA, single nucleotide polymorphisms detection

Recombinant virus production & purificationELISA assaysHPLCMitochondria functional analysis (Oroboros)
Cellular Biology:

Cell isolation, cell culture and co-culture

Organoids culture

Functional studies

Transfection and transduction

Flow cytometry

Histology and Immunohistochemistry:

Tissue fixation/processing: embedding, preparation of blocks & sectioning, staining.

Immunohistochemistry Microscopy Tissue  quantification

Animal:

Husbandry Breeding & screening transgenic mice

Dissections

Blood collection

Surgical procedures

Database management of colony

Physical and cognitive functional assays

Organ and Tissue Physiology:

Making physiological solutions

Tissue Dissection In vitro physiological assays

Electronic Equipment Calibration and Use Completes other duties as assigned.

Reporting Relationships Reports directly to the assigned Principal Investigator Qualifications & Abilities Required High School diploma or equivalent and some basic science courses is required. Excellent oral and written communication skills in English.

Strong interpersonal and organizational skills needed to participate as a creative member of a research team.

Ability to exchange ideas and to work closely with clinicians, physical and occupational therapists, patients and professional organizations.

Ability to timely plan and to coordinate project completions.

Working Conditions Laboratory-based position with substantial time spent conducting experiments at the bench.

Office setting for data analysis, writing, and administrative tasks.

Need for protective gear, such as lab coats, gloves, and safety glasses, depending on the experimental procedures and hazards involved.

The above statements are intended to describe the general nature and level of work being performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all responsibilities,…