Research Biologics Technologist 1

Research Biologics Technologist 1

Shirley Ryan Ability Lab is the global leader in physical medicine and rehabilitation for adults and children with the most severe, complex conditions. By joining our team, you'll be part of our life-changing mission and vision. You'll contribute to an innovative, multifaceted culture that is second to none — one that embraces collaboration, excellence, discovery and compassion. You'll play a role in something that's never been done before as we integrate science and clinical care to help patients achieve better, faster outcomes — as we Advance Human Ability, together.

Principal Responsibilities

Technical:

• Follows pre-defined protocols, policies, procedure, regulations, Good Clinical & Good Laboratory Practices & conducts standard & repetitive experiments & completes basic trouble-shooting techniques.
• Sets-up & operates & maintains standard lab equipment.

Administrative:

• Records test results & may maintain databases and perform some basic statistical analyses.
• Completes standard & routine clerical duties such as copying, filing, data entry, word processing, etc.
• Maintains inventory of reagents, mice & lab supplies.

Skills:

Using an entry level of skill, completes the following activities:

• Molecular Biology and Biochemistry:

  • DNA, RNA, Protein isolation, purification & expression
  • Southern, Northern, Western blotting
  • PCR, RT-PCR,
  • Plasmid preparation
  • Cloning, mapping & sequencing of DNA, single nucleotide polymorphisms detection
  • Recombinant virus production & purification
  • ELISA assays
  • HPLC
  • Mitochondria functional analysis (Oroboros)

• Cellular Biology:

  • Cell isolation, cell culture and co-culture
  • Organoids culture
  • Functional studies
  • Transfection and transduction
  • Flow cytometry

• Histology and Immunohistochemistry:

  • Tissue fixation/processing: embedding, preparation of blocks & sectioning, staining.
  • Immunohistochemistry
  • Microscopy
  • Tissue quantification

• Animal:

  • Husbandry
  • Breeding & screening transgenic mice
  • Dissections
  • Blood collection
  • Surgical procedures
  • Database management of colony
  • Physical and cognitive functional assays

• Organ and Tissue Physiology:

  • Making physiological solutions
  • Tissue Dissection
  • In vitro physiological assays
  • Electronic Equipment Calibration and Use

Completes other duties as assigned.

Reporting Relationships

Reports directly to the assigned Principal Investigator

Qualifications & Abilities Required

• High School diploma or equivalent and some basic science courses is required. Excellent oral and written communication skills in English.
• Strong interpersonal and organizational skills needed to participate as a creative member of a research team.
• Ability to exchange ideas and to work closely with clinicians, physical and occupational therapists, patients and professional organizations.
• Ability to timely plan and to coordinate project completions.

Working Conditions

• Laboratory-based position with substantial time spent conducting experiments at the bench.
• Office setting for data analysis, writing, and administrative tasks.
• Need for protective gear, such as lab coats, gloves, and safety glasses, depending on the experimental procedures and hazards involved.

Pay and Benefits:

Pay Range: $17.05 - $29.62

Benefits:
Shirley Ryan Ability Lab offers a comprehensive benefits program that is competitive with our industry peers in our geographic locations.

Equal Employment Opportunity Employer

Shirley Ryan Ability Lab is an Equal Employment Opportunity Employer. All applicants will be afforded equal employment opportunity without discrimination because of race, color, religion, sex, marital status, national origin or ancestry, citizenship status, age, disability, sexual orientation, gender identity, genetic information, military status, order of protection status, unfavorable discharge from military service, or any other characteristics protected by law.