Research Technologist

Overview

Department: MED-Pharmacology |
Salary/Grade: NEX/13

Job Summary: Participates in design and execution of research lab projects. Conducts experimental tests and procedures, processes and analyzes data, reviews and revises existing research methodologies and may develop new mathematical or computer models for predicting data. May ensure overall lab compliance with all associated rules and regulations.

• The primary focus of the Horiuchi lab is to study the mechanisms of tumor progression in breast cancer and to identify novel targeted treatment strategies. We are particularly interested in understanding the cellular events that influence clinical outcomes in patients with triple‑negative breast cancer (TNBC). Candidates are expected to be fluent in fundamental molecular and cell biological techniques, capable of functioning in a fast‑paced environment, and possess strong organizational skills and operational excellence.

  Key laboratory techniques employed include:

  • Molecular Biology: DNA, RNA, protein isolation, purification and expression;
    Western blotting; PCR, RT‑PCR; plasmid preparation; cloning, mapping and sequencing of DNA; recombinant virus production and purification.
  • Cellular Biology: Cell culture, cell isolation; functional studies; transfection.
  • Histology: Immunohistochemistry; microscopy.
  • Immunology: Flow cytometry; ELISA, ELISpot assay.
  • Animal: Dissections; blood collection; surgical procedures.
  • Administration: Observes experiments, collects, records, summarizes, analyzes, and interprets research data; collates relevant mathematical results; prepares complex tables; draws scientific conclusions; writes portions of grant applications; co‑authors scientific papers.
  • Finance: Negotiates purchases with vendors; verifies accuracy of invoices; orders and maintains inventory of reagents, mice, and lab supplies.
  • Supervision: Trains and directs other lab EEs and/or students in research techniques and procedures.
  • Miscellaneous: Performs other duties as assigned.
• Following overall research objectives, independently designs, plans, conducts and troubleshoots experiments.
• Evaluates protocols and data obtained from groups of experiments and recommends changes to processes, procedures, and techniques to improve quality and validity of results based upon overall project objectives.

• Successful completion of a full 4‑year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years’ lab/bench or other relevant experience required; OR
• Successful completion of a full course of study in an accredited college or university leading to a master’s or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.

• A solid working knowledge of molecular & cell biology techniques, including nucleic acid/protein handling, molecular cloning, gene expression/silencing/editing (CRISPR), and cell culture.
• Multicolor (4+) flow cytometry.
• Animal (mice) handling.

• An advanced training in immunology (with emphasis on the biology of T cells and MDSCs).
• Fluorescence microscopy (confocal).
• Prior in‑depth training in cancer research involving rodent animal models (e.g., transgenic/syngeneic/PDX) is desired.
• A solid understanding of fundamental statistical analysis methods.

Northwestern University is an Equal Opportunity, Affinitive Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.