Sr Res Technologist; Meeks Lab

Job 54159

Location Chicago, Illinois

MED-Urology

NEX/15

Target hiring range for this position will be between be range is as be $27.06-$34.88 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

Conducts complex experimental research in a research lab, functioning as a specialist or technical expert. May manage one or more phase(s) of a major project(s) or all activities of a small project. May ensure overall lab compliance with all associated rules and regulations.

The Meeks Lab is seeking a Scientist who will oversee the operations of the laboratory, develop and manage foundational research projects in bladder cancer, especially the management of complex molecular biology techniques (single cell RNA-seq and digital spatial profiling), oversight of our mouse colony, and help in collaborations with investigators  laboratory is vibrant, collaborative international group, fully supportive of each other to identify cures for bladder cancer.

We are seeking to recruit a new laboratory manager that will make will manage daily operations of a research lab.

• Help to supervise experiments and infrastructure of the lab, including mouse colony management, molecular biology techniques, cell culture, and genetic and protein analyses.
• Directly participate in experiments, and assist other laboratory members with cell culture experiments, single-cell RNA-seq and digital spatial profiling
• Independently plan, perform, and analyze experiments, and collaborate with PI on writing papers and grants;
• Serve as technical resource to, graduate and post-graduate trainees and provide insight, advice and guidance on experimental technologies for cancer research.
• Supervise and train lab support staff and oversee day-to-day operations of the laboratory, develops, implements & administers budgets, grant & administrative procedures.
• Assist in IACUC and laboratory research safety protocols, reviews technical operations ensuring that all processes, protocols & procedures are quality controlled & functioning up to standards

• Provide administrative and operational leadership within the laboratory.
• Independently manage research projects and laboratory priorities.
• Develop and submit research protocols and amendments.
• Maintain and oversee mouse colonies and conduct mouse-related research.
• Perform bioinformatic analysis and interpret research data.
• Communicate scientific findings clearly with investigators, trainees, and collaborators.
• Demonstrate an interest in long-term growth and continuity within the laboratory.

• Acting as a technical expert or specialist in a particular field within a research lab, establishes and optimizes protocols and coordinates conduct of multiple complex experiments.

• DNA, RNA, Protein isolation, purification and expression
• Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Recombinant virus production and purification

• Cell culture, cell isolation
• Functional studies
• Transfection

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry

• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

• Husbandry
• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony

• Oversees and manages lab databases which may include ensuring that experiments are observed and that data is collected and entered correctly.
• Reviews and analyzes data.
• Creates computer models, graphs, reports and summaries for use in publications, professional journals, and grant applications.
• Co-authors scientific papers for presentation and publication.
• Researches and obtains funding.
• Creates and maintains lab manuals regarding operating, safety, and etc. procedures.

• Creates lab financial plan and budget/audit…