Research Technologist 2

Overview

Job : 52524

Location: Chicago, Illinois

Department: MED-Cancer Center

Salary/Grade: NEX/11

Target hiring range: $24.00-$27.80 per hour. Offered salary will be determined by education, experience, knowledge, skills and abilities, as well as internal equity and market data.

Conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.

• Following protocols, designs, sets-up and conducts basic experiments.
• Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.
• Using a fully functioning level of skill, completes the following activities

Molecular Biology:

• DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA Recombinant virus production and purification

Cellular Biology:

• Cell culture, cell isolation Functional studies Transfection

Histology:

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry Microscopy

Immunology:

• Flow cytometry ELISA, Elispot assay Cytokine assay
• Antibody production and purification

Animal:

• Husbandry
• Breeding and screening transgenic mice
• Dissections Blood collection
• Surgical procedures
• Database management of colon

Administration:

• Maintains detailed records of results which may include collecting, extracting & entering data.
• Manipulates, interprets &/or presents results.
• Prepares charts & graphs for presentations & publications
• Performs scientific literature searches in support of research.
• Completes portions of grant applications, manuscripts & publications.

Finance:

• Tracks & summarizes lab supply expenses.
• Verifies accuracy of invoices.
• Orders &/or maintains inventory of reagent, mice &/or lab supplies.

Supervises:

• Trains students.

Miscellaneous:

• Performs other duties as assigned.

• Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience.

• Demonstrated laboratory experience;
• Demonstrated ability to follow detailed instructions as required by research protocols;
• Demonstrated computer skills, including spreadsheets and basic computations;
• Attention to detail and accurate record keeping;
• Ability to work independently and in a team environment;
• Manual dexterity.

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